How long does it take to produce transgenic founders?
You will receive weaned founders six weeks after DNA microinjection.
How long does it take to rederive a mouse line?
You will receive weaned progeny six to eight weeks after in vitro fertilization. This is shorter than a typical quarantine process and the resulting mice are pathogen-free.
I would like to have a cohort of 50 age-matched mice for my study.
We should be able to produce as many as 100 pups within a week of range by in vitro fertilization.
Our male breeders are not breeding and they are getting fat and old. Also, we keep losing newborn mice by cannibalism of the mother.
We may be able to save the line by performing in vitro fertilization, intracytoplasmic sperm injection (ICSI) or round spermatid injection (ROSI) followed by embryo transfer to surrogates of a calm strain.
How do I prepare/submit DNA/ES cells for microinjection?
Please refer to the Protocols page.
My genetics score was "B+" but I'm having a difficulty calculating number of animals needed to produce double knockout mice in my animal protocol.
Please stop by our office any time.