Instrumentation

The main iPSC core facility is situated in a single 800-square foot laboratory comprised of benches, two six-feet tissue culture hoods, four four-feet tissue culture hoods, four in-hood microscopes, three fluorescent inverted microscopes (two Leica and one Olympus), two EVOS microscopes and colony picking stations. There is a state-of-the-art high content cellular imaging system (Molecular Devices, Image Express Micro), along with a liquid handler (Thermo) for handling 96-well and 384-well plates. These systems will be used to develop novel high-content assays to characterize iPSCs and to perform live-cell tracking, live-cell calcium imaging, fluorescent cellular imaging and analysis on differentiated cells from iPSCs. Our state-of-the-art equipment includes microscopes that allow for viewing of live cultures and manipulation of stem cells.

This room is maintained under GLP conditions with positive air pressure, gowning, masks and gloves. It is directly linked to liquid nitrogen storage systems. The iPSC core also has dedicated TC space for growing and differentiating iPS cells produced in the main core facility. To track new cell lines, the core has invested in a bar code labeling system. Other general core facilities within the RMI include a confocal microscope, stereology system, FACS analyzer, gene sequencer and a histology processor. Further cores at Cedars-Sinai include a FACS core with sorter, biostatistics core, genomics core for analyzing iPSC lines at the molecular level and a pathology services for karyotyping.