Contact the RMI iPSC Core for a quote on any of these services.
Please select a service for more information:
Includes generation of clonal iPSC lines for a patient sample provided by customer and Complete or Essential pluripotency characterization package. Please contact us for prices and IRB clearance if an iPSC line needs to be derived from fresh skin biopsy.
|LCLs or peripheral blood||3 clones||Complete||$12,500|
Complete Characterization Package includes: Mycoplasma Testing, AP Staining, ICC for Pluirpotency Markers, G-Band Karyotype, RT-qPCR for Endogenous Pluripotency gene expression, hPSC Scorecard Assay, EBNA1 gDNA PCR, Cell Line Authentication (STR)
Essential Characterization Package includes: Mycoplamsa Testing, AP Staining, ICC for Pluripotent Markers, G-Band Karyotype
Source tissues can include: fibroblast (skin), fresh whole blood (PBMCs), MSCs, adipose cells, various stromal epithelial cell types, and lymphoblastoid cells lines (LCLs or EBV B-cells). The fresh blood must be collected in CPT tubes and centrifuged according to manufacturer's directions. All reprogramming is preformed using non-integrating techniques in defined media (serum and KSR-free) and feeder-free conditions (no MEFs). Cost for each patient line includes the expansion and banking of three thoroughly characterized clonal lines under feeder-free conditions. Each cell line will be accompanied by a rigorous Certificate of Analysis to ensure pluripotency, normal karyotype, embryoid body formation and a variety of services related to the characterization of the pluripotent state (see below).
The RMI iPSC Core offers a variety of assays to ensure the quality and pluripotency of PSC lines. PSC characterization is included in the custom iPSC generation service, or separately as needed. Please contact the iPSC Core for more information on any of our individual characterization services.
|Service||Requirements||Price/ iPSC Line (1 clone)||Price/ iPSC Line (3 clones)|
|Mycoplasma Testing||Please provide the core with at least 1ml of 2-day-old conditioned media. The core regularly performs mycoplasma testing once a month. Contact the core for the next available mycoplasma testing date.||$17||$50|
|Alkaline Phosphatase (AP) Staining||Please provide the core with 1 well of 50-60% confluent cells.||$40||$110|
|Immunocytochemistry (ICC) staining
SSEA4 + OCT4
TRA-1-60 + NANOG
TRA-1-81 + SOX2
|Please proved the core with 1 well of good quality cells that are at least 50-60% confluent.||$80||$225|
|OCT 3/4 + NANOG (or SSEA4) Imaging/Flow Cytometry||6 samples, minimum, are required for running this assay. Price may be split between multiple investigators and Core can help coordinate. Please provide the core with 1 confluent well of each sample.||$120||$330|
|G-Band Karyotype||Please provide the core with 2 80% confluent wells.||$450||$1,250|
|PluriTest Assay — Currently Unavailable||Please provide the core with 1-2 80% confluent wells.||$320||$900|
|RT-qPCR for endogenous/exogenous
pluripotency gene expression
|Please provide the core with 1-2 wells of good quality cells that are at least 80% confluent.||$100||$280|
|Embryoid Body (EB) formation,
RT-PCR gene expression for 3 lineages
|Please provide the core with a 70-80% confluent 6-well plate of cells.||$340||$950|
|hPSC Scorecard Assay||Price is per clone. Please provide the core with a 70-80% confluent 6-well plate of cells. We will generate EBs and also test parent iPSCs. This assay is done in pair of iPSCs + EBs generated from the iPSCs for 10-18 days. Price reflected is for the pair.||$600||$1,500|
|EBNA1 gDNA PCR||Confirm lack of exogenous plasmid presence. Price is per clone.||$200||$550|
|Southern Blot Analysis||Confirm lack of plasmid integration.||$300||$850|
|Teratoma formation||Price per clone. Contact the core for more information regarding teratoma formation.||$2,500||$6,500|
As part of the custom iPSC generation service, the core will expand and bank three fully characterized iPSC clones per cell line. This service is also available to iPSC and ESC lines generated outside of Cedars-Sinai.
|Cell Line Cryopreservation||Guaranteed cryopreservation of high quality, mycoplasma negative cells (6 vials total-feeder free).||Please provide the core with 1 well of starter cells.||$150|
|PSC Thaw||The core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and maintain cells for 1 week.||Please provide the core with 1 vial of cryopreserved cells.||$50|
|PSC Thaw and Expansion||The core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and expand cells into a full 6-well plate over the course of 2 weeks.||Please provide the core with 1 vial of cryopreserved cells.||$150|
|PSC Research Grade Biobank||The core will generate a research grade bank of multiple cryopreserved vials of PSCs.||Karyotype will be performed prior to initiating banking and at the time of creating a research grade lot.||Contact Us|
|Image Express Micro||Independent acquisition and analysis.||User must be trained.||$75/hr|
|Image Express Micro||Staff assisted acquisition and analysis.||User must be trained.||$120/hr|
|hiPSCs or hESCs||Cryovial||Frozen||$650|
|hiPSCs or hESCs||6-well plate||Live Culture||$120 ($20/well) + $650 (cost of vial) = $770|
|Reporter cell lines (iPSCs or ESCs) Nuclear GFP in AAVS1 safe harbor locus||6-well plate||Live Culture||$120|
|Reporter cell lines (iPSCs or ESCs) Nuclear GFP in AAVS1 safe harbor locus||Cryovial||Frozen||$700|
*Shipping will carry an additional cost
Contact the core for custom generation of P1 and P2 non-mitomycin treated MEFs.