Instrumentation

 
 
   

Protein Chemistry

For recombinant protein production, the lab has a GE Pharmacia AKTA Purifier 10 UPC chromatography system, with flow rates of 0.001 to 10ml/min at pressures up to 25 MPa. The system includes a pump with four pump heads in two pump modules, a mixer with a 0.6 ml chamber for precision gradient formation, and a UV/pH/conductivity monitor with fixed wavelength detection and conductivity flow cell. The system is also equipped with one INV-907 injection valve with a 100 microliter sample loop and a UNICORN software package. In addition, a Waters Alliance HPLC System is available for purification of peptides or small drug molecules.

 

Biacore

A SensíQ® Pioneer Research System is available for characterizing protein-ligand interaction. The system includes a three channel, fully-automated SPR Instrument with two well plates(custom and 96 well), temperature control in reaction and sample areas, on-board in-line buffer degassing, host computer and display, gradient injection subsystem, application and control software, and Q-Dat™ data analysis software.

 

X-ray Crystallography

The core is equipped with a Rigaku X-ray diffraction unit for diffraction data analysis from protein crystals. The instrument has an R-AXIS IV++ dual image plate detector, VariMax optics, Micro007 generator and Cobra cryostream for low temperature data collection. R-AXIS IV++ has excellent sensitivity, wide dynamic range, large aperture, and fast readout. The X-ray generator Micro007 produces an intense, monochromatic beam for maximal diffraction intensity and thus allows us to collect data from weakly diffracting crystals. The unique Cobra non-liquid nitrogen cryostream allows to cryo-data collection from 80°-400°K. The unit is attached to a Linux workstation with HKL-3000 data processing software seamlessly interfaced to determine protein structures.

 

Isothermal titration calorimetry (MicroCal VP-ITC) and differential scanning calorimetry (VP-DSC)

The core is equipped with computer and necessary software and is adjacent to our laboratory. The laboratory is equipped with differential scanning calorimetry (GE MicroCal VP-DSC). VP-ITC will enable the study of protein-ligand binding interaction and estimate Kd, ΔG, and ΔH without labeling either protein or ligands. VP-DSC enables estimation of the stability of the complex, which may be used for biologicals.

 

Zatasizer Nano ZS

Our Zatasizer is a desktop particle characterizer based on the principles of dynamic light scattering (DLS). DLS has been interfaced with FPLC to facilitate the separation of mono-dispersed species of recombinant proteins.