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- For non Cedars-Sinai external users, please inquire for price information.
- Lenti/retro vector: 2-3 weeks turnaround time for vector production (for one step cloning custom vector production 3-4 weeks turnaround time). Four plate scale titer is 10e6 - 10e7 in 200 ul.
- Maximum insert size for lenti vector is <4 kb (including promoter, gene, polyA). Sub-optimum (larger size/toxic) inserts will adversely affect the yield.
- Titer will be based on p24 ELISA.
Adeno first generation vector: Maximum insert size is 8 kb. Turnaround time for viral amplification is 2 months.
Custom vector cloning, DNA transfection and viral amplification with gene provided is 3-4 months from the commencement of production. Without the gene provided, timing is 4-5 months.
Users will not be billed until the virus is made. BSL2 biosafety containment required. Users should have biosafety approval through the Institutional Biosafety Committee (IBC).
Lenti Vectors available at vector core
Adeno Shuttle Vectors available at vector core