Homayon Ghiasi, PhD
Director, Ophthalmology Viral Immunology & Vaccine Laboratory, Surgical Research
|Regenerative Medicine Institute|
Awards and Activities
|Editorial Board Member: The Open Virology Journal||Current|
|Editor: Current Eye Research||Current|
|American Society for Microbiology||1985|
|Association for Research in Vision and Ophthalmology||1987|
Research interest concerns viral pathogenesis and immunology of herpes simplex virus type 1 (HSV-1) infection. The specific areas of studies are: (1) The role of HSV-1 in herpes induced corneal scarring; (2) Vaccine development against ocular HSV-1 infection; and (3) The role of viral infection and cytokines in CNS demyelination.
1. Shown that a DNA cocktail of 5 HSV-1 glycoprotein genes is more efficacious than immunization with live virus or protein vaccine.
2. Ocular HSV-1 infection is the leading cause of corneal blindness in U.S. Have shown that HSV-1 induced eye disease is associated with HSV 1 glycoprotein K.
3. As a model for multiple sclerosis (MS), have shown that a combination of HSV-1 and IL-2 over-expression resulted in CNS demyelination.
Current investigations include:
The following NIH funded projects are in progress in the lab: (1) Improvement of vaccine efficacy against HSV. The main goal in this study is to develop a safe and effective vaccine against ocular HSV-1 infection; (2) The role of gK in HSV-1 induce corneal scarring. In this study we are testing the role of HSV-1 glycoprotein K (gK) in HSV-1 induced eye disease; and (3) The role of IL-2 and virus in optic neuritis. This study seeks to elucidate the biological and immunological mechanisms responsible for CNS demyelination in ocularly infected mice.
- Mott KR, Perng GC, Osorio Y, Kousoulas KG, Ghiasi H: A recombinant herpes simplex virus type 1 expressing two additional copies of gK is more pathogenic than wild-type virus in two different strains of mice. J. Virol., 81(23): 12962-72, 2007
- Osorio Y, Mott KR, Jabbar AM, Moreno A, Foster TP, Kousoulas KG, Ghiasi H: Epitope mapping of HSV-1 glycoprotein K (gK) reveals a T cell epitope located within the signal domain of gK. Virus Res., 128(1-2): 71-80, 2007
- Mott K, Brick DJ, van Rooijen N, Ghiasi H: Macrophages are important determinants of acute ocular HSV-1 infection in immunized mice. Invest. Ophthalmol. Vis. Sci., 48(12): 5605-15, 2007
- Mott KR, Osorio Y, Maguen E, Nesburn AB, Wittek AE, Cai S, Chattopadhyay S, Ghiasi H: Role of anti-glycoproteins D (anti-gD) and K (anti-gK) IgGs in pathology of herpes stromal keratitis in humans. Invest. Ophthalmol. Vis. Sci., 48(5): 2185-93, 2007
- Osorio Y, La Point SF, Nusinowitz S, Hofman FM, Ghiasi H: CD8+-dependent CNS demyelination following ocular infection of mice with a recombinant HSV-1 expressing murine IL-2. Exp. Neurol., 193(1): 1-18, 2005
- Koelle DM, Ghiasi H: Prospects for developing an effective vaccine against ocular herpes simplex virus infection. Curr. Eye Res., 30(11): 929-42, 2005