Quantitative PCR (qPCR) Platforms
QuantStudio™ 12K Flex Real-Time PCR System Featuring a much higher throughput:
More automation:
|
QuantStudio™ 12K Flex Real-Time PCR System |
High-Throughput Nanofluidic Single-cell qPCR (BioMark™ HD System) |
BioMark HD System |
Next-Generation Sequencing
Illumina NovaSeq 6000 |
Illumina NovaSeq 6000 |
Illumina NextSeq 500 In a single run, NextSeq 500 can sequence up to:
|
Illumina NextSeq 500 |
Illumina MiSeq |
Illumina MiSeq |
NGS Library Prep
epMotion 5075tc
|
epMotion 5075tc |
10X Chromium The system supports the following applications:
|
10X Chromium |
Fluidigm C1 Single-Cell Auto Prep system
|
Fluidigm C1 Single-Cell Auto Prep system |
Formulatrix Mantis |
Formulatrix Mantis |
Quality Control
Invitrogen E-Gel |
Advanced Analytical Fragment Analyzer |
Agilent Bioanalyzer |
Invitrogen Qubit |
Informatics Infrastructure
Cedars-Sinai uses a Sun Grid Engine high-performance computing grid to analyze next-generation sequencing and for other computational research.
THE QUEUE |
NODE |
CPU |
Genomics queue |
4 nodes |
4 x 16 CPUs (per node) |
LargeMem queue |
4 nodes |
4 x 24 CPUs (per node) |
All/Short/Long/MatLab queue |
370 nodes |
370 x 2 CPUs (per node) |
Virtual Machine Servers
A virtual machine (VM) is a software implementation on the machine that executes programs like a physical machine. We currently have two VM servers:
- VM server 1 (cslxgenomics01): 4 CPUs and 16 Gs for nonparallel programs.
- VM server 2 (cslxgenomics02): 2 CPUs and 8 Gs for web portal, LIMS and databases.
Storage/Backup
The Genomics Core has several layers (levels) of storage devices (fast storage, slower storage) and has access to 200 TB plus.