Cryosection of heart tissue showing mitochondria labeled with fluorescent Timer protein targeted to mitochondria. MitoTimer reveals mitochondrial turnover.
Message from the Core Director
The Metabolism and Mitochondrial Research Core provides comprehensive services for analysis of metabolism and mitochondrial function in the research setting. We hope to create a paradigm shift in which medicine is approached with an awareness of organelle dysfunction underlying organ dysfunction. In the future, we plan to expand services to provide genotype and phenotype diagnostics for inherited mitochondrial diseases and to offer mitochondrial and metabolic phenotyping of clinical samples.
Metabolism and Mitochondrial Research Core Services
The Cedars-Sinai Metabolism and Mitochondrial Research Core provides comprehensive services for analysis of metabolism and mitochondrial function in the research setting. Instrumentation includes Seahorse XFe24 and XFe96 Extracellular Flux Analyzer for measurement of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in order to interrogate changes in oxidative phosphorylation and glycolysis in cells or isolated mitochondria. This bioenergetic analysis allows testing multiple conditions per assay well and direct, simultaneous comparisons across wells, enabling faster throughput with smaller samples compared with traditional respirometry methods. There is also a Seahorse XFp, which is ideal for experiments involving only a few samples or with limiting amounts of material. Like the XFe24 and XFe96, the XFp can monitor oxygen consumption rate and extracellular acidification rate. It is ideal for pairwise comparisons, bioenergetic phenotyping and analysis of patient-derived samples (e.g., biopsies). The Seahorse work area (200 square feet) also includes a biosafety cabinet and tissue culture incubator for cell culture plates, to be used with the Seahorse instruments. A Keyence BZ-9000 fluorescence microscope with user-friendly software for rapid image acquisition and advanced functions including 3-D deconvolution, image stitching and quantitative image analysis is also available through the core.
Core services are accessed on a fee-for-service basis and consultations are available at no charge.