Spectrum of mitochondrial morphologies in H9C2 cardiomyocytes.
Message from the Core Director
The Mitochondria, Metabolism and Cardiac Phenotyping Core provides comprehensive services for analysis of metabolism and mitochondrial function in the research setting. New services include central carbon chain metabolomics and a mass spec-based quantitation of approximately 30 proteins involved in glycolysis and oxidative phosphorylation, the MitoPlex (available for mouse and human in June 2018). The core team hopes to create a paradigm shift in which medicine is approached with an awareness of organelle dysfunction underlying organ dysfunction. Additionally, we are now offering mouse cardiac phenotyping services including echocardiography and surgical models including myocardial infarction and pressure overload.
Mitochondria, Metabolism and Cardiac Phenotyping Core Services
Instrumentation in the Mitochondria, Metabolism and Cardiac Phenotyping Core includes Seahorse XFe24 and XFe96 Extracellular Flux Analyzer for measurement of oxygen consumption rate and extracellular acidification rate in order to interrogate changes in oxidative phosphorylation and glycolysis in cells or isolated mitochondria. This bioenergetic analysis allows testing multiple conditions per assay well and direct, simultaneous comparisons across wells, enabling faster throughput with smaller samples compared with traditional respirometry methods. There is also a Seahorse XFp, which is ideal for experiments involving only a few samples or with limiting amounts of material. Like the XFe24 and XFe96, the XFp can monitor oxygen consumption rate and extracellular acidification rate. It is ideal for pairwise comparisons, bioenergetic phenotyping and analysis of patient-derived samples (e.g., biopsies).
The Seahorse work area (250 square feet total) includes a biosafety cabinet and tissue culture incubator for cell culture plates to be used with the Seahorse instruments. Core services are accessed on a fee-for-service basis and initial consultations are available at no charge. We are pleased to begin offering services for mouse cardiac phenotyping, which includes select surgical procedures and echocardiogram assessment of outcomes.
The MitoPlex is a mass spectrometry-based quantitation of approximately 30 proteins in glycolysis and oxidative phosphorylation (see diagram below) that can complement metabolomics and Seahorse respirometry to provide a detailed portrait of bioenergetic pathways in cells and tissues.