Instrumentation

Seahorse XFe24 and XFe96

This instrument measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in order to interrogate oxidative phosphorylation, glycolysis and fatty acid oxidation. It has a 24-well capacity per plate for examining cells or isolated mitochondria. This bioenergetic analysis allows testing multiple conditions per assay well and direct, simultaneous comparisons across wells, enabling faster throughput with smaller samples compared to traditional respirometry methods. The Seahorse work area also includes a biosafety cabinet and tissue culture incubator for preparing cell culture plates to be used with the Seahorse instruments.

Seahorse XFp

This smaller 8-well capacity instrument is ideal for experiments involving only a few samples or with limiting amounts of material. Like the XF24, the XFp monitors OCR and ECAR. It is ideal for pairwise comparisons, bioenergetic phenotyping and analysis of patient-derived samples (e.g., biopsies).

FUJIFILM VisualSonics Vevo 3100 Preclinical Imaging System

The Vevo 3100 is a powerful, advanced micro-ultrasound imaging system offering reduced speckle noise and artifacts for monitoring small animal models. Its easy-to-use touch screen interface and customizable workflow settings allows for rapid high-throughput acquisition of anatomical, hemodynamic, functional and molecular information.

SCIEX QTRAP 6500 and Agilent Triple Quad Mass Spectrometers

The mitoplex protein assay and metabolite assays are performed on SCIEX QTRAP 6500 and Agilent Triple Quad Mass Spectrometers, respectively. Each is coupled to a high-pressure liquid chromatography set up. The instruments are tuned to collect data on only the analytes of interest, optimizing sensitivity, specificity and quantitative accuracy.

Keyence BZ-9000 Fluorescence Microscope

This easy-to-use fluorescence microscope uses advanced software for image acquisition and data analysis. Three-D deconvolution, image stitching and quantitative image analysis are straightforward. Users often perform cell counting by nuclear staining after performing Seahorse analyses to normalize data to cell number.