Light Microscopy & Image Analytics
Multiple scanning and imaging devices feature state-of-the-art technology, where researchers can analyze and generate an array of digital images from millions of tissue and blood samples to advance scientific discovery.
Some of our capabilities include:
- Imaging of immunohistochemistry (IHC) and fluorescent stains
- Live cell imaging
- Image annotation
- Image analysis of multiplex IHC and fluorescent stains
- Morphometric measurements
Research pathologists are available for pathology review of stained slides and for annotation of regions of interest for image analysis.
- Imaging of human and animal cells and tissues on glass slides
- Confocal and light sheet microscopy
Imaging Analysis Services
- Digital quantification analysis of fluorescence and brightfield images, as well as slide scans with single or multiple probes
- Web-based interface to access, view and analyze images
Imaris (Bitplane) is designed to provide automated quantification of fluorescent 3-dimensional image analysis. With the most updated version, iMaris can perform isosurface rendering, object detection/counting, filament tracing/particle tracking, quantitative co-localization and distance measurements that are extremely useful for a variety of biological experiments and applications.
iMaris supports most microscope formats on the market including images collected by Zeiss, Leica and Olympus acquisition software.
Nanostring GeoMX Digital Spatial Profiling (DSP)
GeoMx Digital Spatial Profiler is a novel platform developed by NanoString. Digital Spatial Profiling is based on nCounter® barcoding technology and enables a spatially resolved digital readout of up to 96 proteins or RNA targets in a multiplexed assay. The assay relies upon antibody or RNA probes coupled to photocleavable oligonucleotide tags. After simple-step hybridization of probes to slide-mounted formalin fixed paraffin-embedded (FFPE) tissue sections, the oligonucleotide tags from ROI are released by UV exposure and quantitated in a standard nCounter assay, yielding a spatially resolved digital profile of analyte abundance. Given this novel technology, one can easily generate data from FFPE tissue with multiplex protein/RNA detection, quantitative (nonamplified) readout, and high throughput.
- Quantify up to 96 proteins and over 1000 RNA targets with spatial context
- Process 10-20 slides/day with readout on the nCounter® System or with NGS
- Preserve precious samples with nondestructive processing
Have Questions or Need Help?
Contact us if you have questions or would like to learn more about the Biobank and Research Pathology Resource at Cedars-Sinai.
Spielberg, Room 110
8723 W. Alden Drive
Los Angeles, CA 90048