The NovaSeq 6000 system offers flexibility for virtually any sequencing method, genome and scale. Patterned flow cell technology generates an unprecedented level of throughput for a broad range of sequencing applications. Four flow cells are available, as well as the NovaSeq XP workflow that allows for the loading of individual lanes with different library types. Up to 3000 Gb output, 10 B reads and 2x150 bp read length.
The NextSeq 500 system delivers the power and flexibility to carry out applications such as whole genome, exome, transcriptome and methylation sequencing, among others. Two flow cell configurations allow for tunable outputs. Up to 120 Gb output, 400 M reads and 2x150 bp read length.
The MiSeq system offers the first end-to-end sequencing solution, integrating cluster generation, amplification, sequencing and data analysis into a single instrument. The MiSeq is best suited for whole genome sequencing of smaller model organism genomes, medium to small targeted panels, amplicon sequencing, 16S metagenomics, quality control of libraries, and more. Flow cells are available in three sizes. Up to 15 Gb output, 25 M reads and 2x300 bp read length.
Single Cell Platforms
The 10x Genomics Chromium Controller performs high-throughput, parallel partitioning and automated barcoding for powerful RNA and epigenomics sequencing applications for up to 80,000 single cells in a single run. Single cell transcriptomics applications include gene expression profiling, simultaneous gene and cell surface protein expression profiling, gene expression CRISPR screening, immune profiling, simultaneous immune and cell surface protein profiling, and immune profiling and antigen specificity. Combine single cell transcriptomics with single cell chromatin accessibility for a multi-omics approach.
Explore mutational diversity on a cell-by-cell basis using the Tapestri single cell targeted DNA-Seq platform. Detect SNVs and CNVs in the same cell, or DNA and protein for a multi-omics approach. The Tapestri platform can be used to detect rare subclones (down to 0.1%), identify true mutation co-occurrence in clonal populations, resolve zygosity and reconstruct phylogenetic lineages. Compatible with fresh or frozen cells or tissue, as well as methanol fixed cells. Capture from 5,000-10,000 cells. Predesigned and custom panels available.
Automated Liquid Handlers
The Chromium Connect automates 10x Genomics’ scRNA-Seq assay, going from single cells to sequencing-ready libraries with minimal sample handling using an integrated and validated solution. Reduce errors from manual pipetting and minimize technical variation to generate consistent and reproducible single cell gene expression results across experiments, users and sites. Run up to eight samples in parallel and capture from 500-10,000 cells per sample.
With a dispense range from 100 nl and a 6 µL dead volume, this non-contact liquid dispenser provides ultimate flexibility for single-cell genomics applications, and assay miniaturization and optimization. The MANTIS utilizes positive displacement dispensing technology to measure and dispense discrete volumes of liquid with high accuracy and speed. The user-friendly software allows for quick setup and dispensing into 96-, 384- or 1536-well plates.
The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of DNA and RNA. Together with the 2100 Expert Software and Bioanalyzer assays, the 2100 Bioanalyzer provides highly precise analytical evaluation of various samples types in many workflows, including next-generation sequencing (NGS), gene expression, biopharmaceutical and gene editing research.
The Agilent 4200 TapeStation system is an automated electrophoresis tool for DNA and RNA sample quality control. Fully automated sample processing enables the unattended analysis of size, concentration and integrity. Analyze any sample number between 1 and 96. The TapeStation system provides a complete solution for true end-to-end sample quality control within any next-generation sequencing workflow.
Fully automate and standardize your tissue dissociation or homogenization of up to eight samples simultaneously. Tissue dissociation is performed in a closed and sterile system. Ready-to-use programs, in conjunction with Miltenyi tissue dissociation kits that combine mechanical dissociation and enzymatic treatment, yield single-cell suspensions with excellent viability and preserved epitope integrity. Use the gentleMACSTM Octo Dissociators With Heaters upstream of your single cell genomics analyses.
Automated, reproducible, hands-off tissue dissociation from fresh, frozen or OCT-embedded tissue into single cells or single nuclei. Choose from a selection of automated preset protocols and preformulated reagents, or create your own protocols and use your reagents of choice. The Singulator allows researchers to reproducibly prepare suspensions of high viability cells or nuclei in high yield and from small samples. Use the Singulator 100 upstream of your single cell genomics analyses.
The autoMACS® Pro Separator, in combination with Miltenyi MACS® MicroBeads, provide fast, reproducible, automated cell isolation of virtually any cell type. The Pro Separator is compatible with automated or manual cell labeling, with the ability to process up to six samples in a single run. MACS® MicroBeads offer cell separation with maximal purity and recovery, with no cell stress and high cell viability using column-based magnetic cell isolation. Use the autoMACS® Pro Separator and MACS® MicroBeads upstream of your single cell genomics analyses.
The MACSQuant® Tyto Sorter offers a revolutionary way to sort your cells. Unlike during conventional droplet sorting procedures, cells do not experience high pressure, are not decompressed and no charge is applied, resulting in great viability and functionality of cells. Samples are kept contamination-free within the disposable, fully closed MACSQuant® Tyto Cartridge, with no risk of carry-over or contamination of fluidics. The sorter is equipped with three lasers, allowing for 10-parameter cell sorting. Use the MACSQuant® Tyto Sorter upstream of your single cell genomics analyses.
The MACSQuant® Analyzer 16 Flow Cytometer offers revolutionary automation of flow cytometers. Fully automated cell staining, data acquisition and data analysis. Automated sampling can be achieved from single tubes, multiple tubes or a 96-well plate. Select from 14 fluorescence channels. Use the MACSQuant® Analyzer 16 Flow Cytometer upstream of your single cell genomics analyses.
The Covaris focused-ultrasonicator uses the Adaptive Focused Acoustics® (AFA) process to deliver high frequency, accurately focused and specifically targeted acoustic energy to samples for a variety of application areas including: DNA shearing for next-generation sequencing, RNA and protein extraction, chromatin shearing and cell lysis. The characteristics of the instrument enable a highly reproducible and isothermal process, reducing experimental variation and heat-induced damage to the sample.
Real-time PCR Systems
The QuantStudio 12K Flex Real-Time PCR System is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The system includes easy-to-change blocks that can accommodate OpenArray plates, TaqMan array cards, 384-well and 96-well plates.
Nucleic Acid Quantification Systems
Benchtop fluorometers designed to accurately measure DNA, RNA, and protein quantity with more sensitivity and accuracy than UV absorbance.
High throughput efficiency and accuracy. The Nano Drop 8000 delivers full-spectrum, micro-volume UV-Vis absorbency measurements for up to eight samples at one time.
Have Questions or Need Help?
Contact us if you have questions or would like to learn more about the Applied Genomics, Computation & Translational Core at Cedars-Sinai.
8723 Alden Drive
Steven Spielberg Building, Room 303
Los Angeles, CA 90048